Symptoms of the disease include necrotized leaves and rotted bulbs ( Gitaitis and Gay, 1997). Onion center rot is an economically impactful disease that routinely results in significant losses to the yield and marketability of onion ( Allium cepa L.). A combination of assays that include PCR of virulence genes and an RSN assay can potentially aid in identification of onion-bulb-rotting pathogenic P. ananatis strains, which cause extensive bulb necrosis in a neck-to-bulb infection assay compared to alt negative P. The HiVir gene cluster is required to induce necrosis on onion tissues via synthesis of pantaphos, (2-hydroxy P. Previous genomic studies identified two virulence gene clusters, HiVir and alt, associated with center rot. Pantoea ananatis is a gram-negative bacterium and the primary causal agent of center rot of onions in Georgia. 4Departamento de Producción Vegetal, Centro Regional Sur (CRS), Facultad de Agronomía, Universidad de la República, Canelones, Uruguay.3Área de Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay.2Centre for Microbial Ecology and Genomics, Forestry and Agriculture Biotechnology Institute, Department of Biochemistry, Genetics and Microbiology, University of Pretoria, Pretoria, South Africa.1Department of Plant Pathology, University of Georgia College of Agricultural and Environmental Sciences, Athens, GA, United States.Severns 1, Teresa Coutinho 2, Bhabesh Dutta 1 and Brian H. Stice 1, Gi Yoon Shin 2, Stefanie De Armas 3, Santosh Koirala 1, Guillermo A.
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